Abstract
By using the selective capture of transcribed sequences (SCOTS) approach, we identified 28 genes preferentially expressed by the major swine pathogen and zoonotic agent Streptococcus suis upon interaction with porcine brain microvascular endothelial cells. Several of these genes may be considered new S. suis candidate virulence factors. Results from this study demonstrate the suitability of SCOTS for the elucidation of gene expression in streptococcal species and may contribute to a better understanding of the pathogenesis of S. suis infections.
Publication types
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Research Support, Non-U.S. Gov't
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Validation Study
MeSH terms
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Animals
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Base Sequence
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Brain / blood supply*
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Brain / microbiology*
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Cell Line
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DNA Primers / genetics
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DNA, Bacterial / genetics
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Endothelial Cells / microbiology*
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Fimbriae, Bacterial / genetics
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Gene Expression
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Genes, Bacterial*
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Genetic Techniques
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Genomic Islands
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Humans
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Polymerase Chain Reaction
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Streptococcal Infections / microbiology
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Streptococcal Infections / veterinary
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Streptococcus suis / genetics*
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Streptococcus suis / pathogenicity*
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Swine
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Swine Diseases / microbiology
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Transcription, Genetic
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Virulence / genetics
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Zoonoses / microbiology
Substances
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DNA Primers
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DNA, Bacterial