Abstract
Using phenotypic approaches, we have detected that 17% of human intestinal Lactobacillus and Bifidobacterium strains could be exopolysaccharide (EPS) producers. However, PCR techniques showed that only 7% harbored genes related to the synthesis of heteropolysaccharides. This is the first work to screen the human intestinal ecosystem for the detection of EPS-producing strains.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Amino Acid Sequence
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Bifidobacterium / classification
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Bifidobacterium / genetics
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Bifidobacterium / isolation & purification*
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Bifidobacterium / metabolism*
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Ecosystem
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Genes, Bacterial
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Glycosyltransferases / genetics
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Glycosyltransferases / metabolism
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Humans
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Intestines / microbiology*
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Lactobacillus / classification
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Lactobacillus / genetics
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Lactobacillus / isolation & purification*
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Lactobacillus / metabolism*
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Molecular Sequence Data
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Phenotype
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Phylogeny
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Polymerase Chain Reaction
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Polysaccharides, Bacterial / biosynthesis*
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Polysaccharides, Bacterial / genetics
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Sequence Homology, Amino Acid
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Species Specificity
Substances
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Polysaccharides, Bacterial
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Glycosyltransferases