The study of trabecular structures is important for understanding the mechanism of alcohol related to bone changes. Alcohol consumption can compromise the body mineral composition, affecting the bone metabolism and compromising the skeleton. The effects of the ethanol treatment on the internal microarchitecture of bone samples through 3D microcomputed tomography are shown in this study. The data was acquired from a radiographic system with a micro focus X-ray conic beam and it was used the Feldkamp's technique was used to carry out the 3D reconstructions. The measured microstructure parameters, which were based on stereological concepts, were bone volume fraction, relationship between bone surface and volume, trabecular number, separation and thickness. The results show that this technique is able to analyze these kinds of structures, especially rat bone, as these structures in rats (trabecular diameter) are thinner than in human bones.