[Analysis of Foxp3+ CD4+ CD25+ regulatory cells in peripheral blood of patients with SLE]

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2007 May;23(5):432-5.
[Article in Chinese]

Abstract

Aim: To investigate the expression of GITR on T cells and the expression of Foxp3(+) CD4(+) CD25(+) regulatory T cells in the peripheral blood of patients with SLE.

Methods: The expression of GITR and Foxp3(+) CD4(+) CD25(+) regulatory T cells in the peripheral blood was determined by flow cytometry(FCM).

Results: Compared with health control, the expression of Foxp3(+) CD4(+) CD25(+) regulatory T cells in peripheral blood from SLE patients was lower (P<0.05), but there is no difference in the expression of Foxp3(+) CD4(+) CD25(+) regulatory T cells between active and inactive SLE patients (P>0.05). GITR expression on both CD3(+) CD4(+) T cells and CD3(+) CD8(+) T cells of SLE patients increased significantly (P>0.05). With the development of SLE activity, GITR expression didn't change significantly on both CD3(+) CD4(+) T cells (P>0.05) and CD3(+) CD8(+) T cells (P>0.05).

Conclusion: The expression abnormality of both Foxp3(+) CD4(+) CD25(+) regulatory cells and GITR may play important role in the imbalance of immune homeostasis in SLE.

MeSH terms

  • Adolescent
  • Adult
  • CD3 Complex / immunology
  • CD4 Antigens / immunology*
  • CD4-Positive T-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes / immunology
  • Female
  • Flow Cytometry
  • Forkhead Transcription Factors / immunology*
  • Glucocorticoid-Induced TNFR-Related Protein
  • Humans
  • Interleukin-2 Receptor alpha Subunit / immunology*
  • Lupus Erythematosus, Systemic / blood*
  • Lupus Erythematosus, Systemic / immunology*
  • Male
  • Middle Aged
  • Receptors, Nerve Growth Factor / metabolism
  • Receptors, Tumor Necrosis Factor / metabolism
  • T-Lymphocyte Subsets / immunology
  • T-Lymphocytes, Regulatory / immunology*
  • Young Adult

Substances

  • CD3 Complex
  • CD4 Antigens
  • FOXP3 protein, human
  • Forkhead Transcription Factors
  • Glucocorticoid-Induced TNFR-Related Protein
  • Interleukin-2 Receptor alpha Subunit
  • Receptors, Nerve Growth Factor
  • Receptors, Tumor Necrosis Factor
  • TNFRSF18 protein, human