Abstract
Hexane, chloroform, ethyl acetate, methanol and water extracts from the flower of Cassia fistula (an ethnomedicinal plant) were tested against bacteria and fungi. All the extracts exhibited antibacterial activity against Gram-positive organisms with minimum inhibitory concentrations (MIC) between 0.078 and 2.5 mg/ml. Among the Gram-negative bacteria, only Pseudomonas aeruginosa was susceptible to the extracts. Ethyl acetate crude extract was fractionated using chromatographic techniques. A crystal was isolated, which was confirmed as 4-hydroxy benzoic acid hydrate using X-ray crystallography. It exhibited antifungal activity against Trichophyton mentagrophytes (MIC 0.5 mg/ml) and Epidermophyton floccosum (MIC 0.5 mg/ml).
MeSH terms
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Acetates / chemistry
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Anti-Bacterial Agents / chemistry
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Anti-Bacterial Agents / isolation & purification
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Anti-Bacterial Agents / pharmacology*
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Antifungal Agents / chemistry
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Antifungal Agents / isolation & purification
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Antifungal Agents / pharmacology*
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Cassia / chemistry*
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Chloroform / chemistry
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Crystallization
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Epidermophyton / drug effects
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Epidermophyton / growth & development
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Ethnopharmacology
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Flowers / chemistry
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Gram-Positive Bacteria / drug effects
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Gram-Positive Bacteria / growth & development
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Hexanes / chemistry
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Hydroxybenzoates / chemistry
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Hydroxybenzoates / isolation & purification
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Hydroxybenzoates / pharmacology
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India
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Methanol / chemistry
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Microbial Sensitivity Tests
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Molecular Structure
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Plant Extracts / chemistry
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology*
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Plants, Medicinal / chemistry
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Pseudomonas aeruginosa / drug effects
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Pseudomonas aeruginosa / growth & development
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Trichophyton / drug effects
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Trichophyton / growth & development
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Water / chemistry
Substances
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Acetates
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Anti-Bacterial Agents
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Antifungal Agents
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Hexanes
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Hydroxybenzoates
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Plant Extracts
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Water
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ethyl acetate
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Chloroform
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Methanol