Comparison of an immunochromatographic rapid test with enzyme-linked immunosorbent assay and polymerase chain reaction for the detection of Shiga toxins from human stool samples

Katharina Grif; Dorothea Orth; Manfred P Dierich; Reinhard Würzner

doi:10.1016/j.diagmicrobio.2007.03.022

Comparison of an immunochromatographic rapid test with enzyme-linked immunosorbent assay and polymerase chain reaction for the detection of Shiga toxins from human stool samples

Diagn Microbiol Infect Dis. 2007 Sep;59(1):97-9. doi: 10.1016/j.diagmicrobio.2007.03.022. Epub 2007 May 29.

Authors

Katharina Grif¹, Dorothea Orth, Manfred P Dierich, Reinhard Würzner

Affiliation

¹ Department of Hygiene, Microbiology and Social Medicine, Innsbruck Medical University, Austrian Reference Centre for Enterohemorrhagic Escherichia coli, A-6020 Innsbruck, Austria. [email protected]

PMID: 17532589
DOI: 10.1016/j.diagmicrobio.2007.03.022

Abstract

Rapid detection of enterohemorrhagic Escherichia coli is important for its successful treatment. We have evaluated the immunochromatographic Duopath Verotoxin-test for detection of Shiga toxins, in comparison with enzyme-linked immunosorbent assay and polymerase chain reaction, on 240 clinical human stool samples. The Duopath-test showed a lower sensitivity and specificity.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Chromatography / methods
Enzyme-Linked Immunosorbent Assay
Escherichia coli Infections / diagnosis*
Feces / microbiology*
Humans
Immunoassay / methods*
Polymerase Chain Reaction
Predictive Value of Tests
Shiga Toxins / analysis*

Substances

Shiga Toxins