Primary amides as selective inhibitors of cathepsin K

Bioorg Med Chem Lett. 2007 Aug 1;17(15):4328-32. doi: 10.1016/j.bmcl.2007.05.024. Epub 2007 May 16.

Abstract

The nitrile warhead used in a series of cathepsin K inhibitors can be replaced by a less electrophilic primary amide. The accompanying loss of potency can be partially recovered by introducing a substituent alpha to the amide. The potency gain resulting from this addition is not achieved with the nitrile derivatives due to a different geometry of the cysteine adduct in the enzyme active site. This study led to the identification of the primary amide 2g, which is an inhibitory substrate, with an IC(50) of 10 nM against cathepsin K and excellent selectivity versus the other cathepsins.

MeSH terms

  • Amides / chemistry
  • Amides / pharmacology*
  • Cathepsin K
  • Cathepsins / antagonists & inhibitors*
  • Chromatography, High Pressure Liquid
  • Cysteine Proteinase Inhibitors / chemistry
  • Cysteine Proteinase Inhibitors / pharmacology*
  • Models, Molecular
  • Stereoisomerism

Substances

  • Amides
  • Cysteine Proteinase Inhibitors
  • Cathepsins
  • Cathepsin K