Abstract
Lipoprotein e (P4) of Haemophilus influenzae is a phosphomonoesterase, encoded by the hel gene, that has been implicated in the acquisition of heme by this fastidious organism. However, lipoprotein e (P4) is also involved in the utilization of NAD and NMN. Some reports have concluded that the reported heme-related growth defect actually reflects a growth defect for NAD. In the current study, hel insertion mutants were constructed and a role for e (P4) in heme acquisition was demonstrated independent of its role in NAD or NMN acquisition. In addition, a rat model of infection demonstrated a role for e (P4) in the pathogenesis of invasive disease.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bacteremia / microbiology
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Bacteremia / physiopathology
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Bacterial Outer Membrane Proteins / genetics
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Bacterial Outer Membrane Proteins / metabolism*
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Culture Media
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Disease Models, Animal
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Esterases / genetics
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Esterases / metabolism*
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Female
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Haemophilus Infections / microbiology
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Haemophilus Infections / physiopathology
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Haemophilus influenzae / growth & development
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Haemophilus influenzae / metabolism*
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Haemophilus influenzae / pathogenicity*
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Heme / metabolism*
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Humans
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Lipoproteins / genetics
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Lipoproteins / metabolism*
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Pregnancy
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Rats
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Rats, Sprague-Dawley
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Specific Pathogen-Free Organisms
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Virulence
Substances
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Bacterial Outer Membrane Proteins
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Culture Media
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Lipoproteins
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e(P4) lipoprotein, Haemophilus influenzae
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Heme
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Esterases