Oligodeoxynucleotides incorporating structurally simple 5-alkynyl-2'-deoxyuridines fluorometrically respond to hybridization

Org Biomol Chem. 2007 Jun 21;5(12):1845-8. doi: 10.1039/b705805e. Epub 2007 May 16.

Abstract

Selected 5-ethynyl derivatives of 2'-deoxyuridine are shown to fluorometrically respond to hybridization and selectively base-pair to adenine whilst maintaining duplex stability.

MeSH terms

  • Adenine / chemistry
  • Alkynes / chemistry*
  • Base Pairing
  • DNA / chemistry*
  • Deoxyuridine* / analogs & derivatives
  • Deoxyuridine* / chemical synthesis
  • Deoxyuridine* / chemistry
  • Fluorescence
  • Fluorescent Dyes* / chemical synthesis
  • Fluorescent Dyes* / chemistry
  • Hot Temperature
  • Nucleic Acid Denaturation
  • Nucleic Acid Hybridization*
  • Oligonucleotides* / chemical synthesis
  • Oligonucleotides* / chemistry

Substances

  • Alkynes
  • Fluorescent Dyes
  • Oligonucleotides
  • DNA
  • Adenine
  • Deoxyuridine