Extraordinary cross-reactivity of an autoimmune T-cell receptor recognizing specific peptides both on autologous and on allogeneic HLA class II molecules

Tissue Antigens. 2007 Jul;70(1):42-52. doi: 10.1111/j.1399-0039.2007.00849.x.

Abstract

A T-cell receptor's (TCR) recognition of a human leukocyte antigen (HLA)-peptide complex (pHLA) is normally described as being restricted by the HLA molecule and specific for the peptide. This is, however, not always true. Several TCRs have been described, which cross-react with other peptides bound to the restricting HLA molecule. This phenomenon has been considered a variant of molecular mimicry and is suggested to be one of the mechanisms behind autoimmunity. The positive selection of T cells in the thymus imposes low-affinity recognition of the TCRs toward self-pHLA, which increases the probability of the TCR to be promiscuous by nature, and further implies that the T-cell repertoire contains TCRs prone to be autoreactive and thus able to induce autoimmunity. We present an autoimmune TCR showing extreme cross-reactivity to several pHLA comprising both own HLA class II restriction element and allogeneic HLA class II restriction elements in complex with both self-derived and microbially derived peptides. The existence of such a significant cross-reactivity in the context of distinct HLA-DR molecules might be more common among autoimmune TCRs than previously anticipated and potentially reveals a new way of designing altered peptide ligands for therapeutic use.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autoimmunity / immunology*
  • Cell Line, Transformed
  • Cell Transformation, Viral
  • Cross Reactions
  • HLA Antigens / immunology*
  • HLA Antigens / metabolism*
  • Herpesvirus 4, Human / physiology
  • Histocompatibility Antigens Class II / immunology
  • Histocompatibility Antigens Class II / metabolism
  • Humans
  • Hybridomas / immunology
  • Molecular Mimicry / genetics
  • Molecular Mimicry / immunology
  • Peptides / genetics
  • Peptides / immunology*
  • Peptides / isolation & purification
  • Peptides / metabolism*
  • Protein Binding
  • Receptors, Antigen, T-Cell / analysis*
  • Receptors, Antigen, T-Cell / genetics
  • Receptors, Antigen, T-Cell / immunology
  • Receptors, Antigen, T-Cell / metabolism

Substances

  • HLA Antigens
  • Histocompatibility Antigens Class II
  • Peptides
  • Receptors, Antigen, T-Cell