Abstract
Rats received a unilateral lesion of the nucleus basalis by infusion of ibotenic acid. Two weeks after the lesion, osmotic minipumps were implanted, that infused 1 microgram human recombinant nerve growth factor (NGF) or cytochrome-C per day into the lateral ventricle. After four weeks of treatment, release of acetylcholine was measured in the frontal neocortex by means of in-vivo microdialysis. Release was decreased by 75% on the lesioned side; perfusion with 100 mM KCl increased release on the intact side by 130% and on the lesioned side by 80%. Treatment with NGF increased release on the lesioned side twofold, but had no effect on release on the intact side.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acetylcholine / metabolism*
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Animals
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Cerebral Cortex / drug effects
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Cerebral Cortex / physiology*
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Choline / metabolism
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Choline O-Acetyltransferase / metabolism*
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Dialysis / methods
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Functional Laterality
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Hippocampus / drug effects
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Hippocampus / physiology
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Male
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Nerve Growth Factors / pharmacology*
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Parietal Lobe / drug effects
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Parietal Lobe / physiology
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Potassium Chloride / pharmacology
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Rats
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Rats, Inbred F344
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Recombinant Proteins / pharmacology
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Substantia Innominata / physiology*
Substances
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Nerve Growth Factors
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Recombinant Proteins
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Potassium Chloride
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Choline O-Acetyltransferase
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Choline
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Acetylcholine