Abstract
The TSP1/CD36/CD47-complex is involved in T cell expansion and inflammatory responses to beta-amyloid, both relevant to IBM. We report on the mRNA and protein expression of TSP1/ CD36 /CD47-complex in IBM muscles and in human myoblasts after cytokine stimulation. The TSP1/CD36 /CD47 was upregulated in IBM. TSP1 immunolocalized to the connective tissue contiguous to inflammation and CD36/CD47 on the myofibers and CD8+ cells. Further, TNF-alpha upregulated the production of TSP1 and CD47 by myoblasts. The TSP-complex is another inflammatory mediator associated with chronic inflammation in IBM that may perpetuate the immune responses to local antigens in response to TNF-alpha.
Publication types
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Research Support, N.I.H., Intramural
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Research Support, Non-U.S. Gov't
MeSH terms
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CD36 Antigens / genetics
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CD36 Antigens / metabolism*
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CD47 Antigen / genetics
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CD47 Antigen / metabolism*
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Fibroblasts / drug effects
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Fibroblasts / metabolism
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Humans
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Immunohistochemistry / methods
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Muscle, Skeletal / drug effects
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Muscle, Skeletal / metabolism
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Myositis, Inclusion Body / metabolism*
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Myositis, Inclusion Body / pathology
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Myositis, Inclusion Body / physiopathology*
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RNA, Messenger / biosynthesis
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Reverse Transcriptase Polymerase Chain Reaction / methods
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Statistics, Nonparametric
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Thrombospondin 1 / genetics
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Thrombospondin 1 / metabolism*
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Tumor Necrosis Factor-alpha / pharmacology
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Up-Regulation / drug effects
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Up-Regulation / physiology*
Substances
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CD36 Antigens
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CD47 Antigen
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RNA, Messenger
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Thrombospondin 1
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Tumor Necrosis Factor-alpha