Abstract
1. In a previous study, we demonstrated that heat shock augments vascular contractility through the stress response. 2. The current study was designed to identify differentially expressed genes after heat shock by using a novel annealing control primer (ACP) system, which was developed recently to identify authentic genes. 3. Rat aortic rings were mounted in organ baths, exposed to 42 degrees C for 45 min and harvested 4 h after the end of heat shock. Total RNA were used for amplification by the reverse transcriptase-polymerase chain reaction (RT-PCR) with ACP system. Differentially amplified PCR products were sequenced, searched against the GenBank and confirmed by RT-PCR. 4. Genes for connective tissue growth factor, stress-inducible protein 1 and heat shock protein 25 were upregulated, whereas a gene for interferon regulatory factor 1 was downregulated. Immunohistochemistry revealed upregulation of the phosphorylated form of Hsp25 in aortic rings after heat shock. 5. These results suggest that phosphorylated Hsp25 plays a pivotal role in the augmentation of vascular contraction after heat shock.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Aorta, Thoracic / metabolism*
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Aorta, Thoracic / physiopathology
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Connective Tissue Growth Factor
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Databases, Genetic
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Gene Expression Profiling / methods
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Gene Expression*
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HSP27 Heat-Shock Proteins
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Heat Stress Disorders / genetics*
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Heat Stress Disorders / metabolism
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Heat Stress Disorders / physiopathology
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Heat-Shock Proteins / genetics
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Heat-Shock Proteins / metabolism
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Hot Temperature*
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Immediate-Early Proteins / genetics
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Immediate-Early Proteins / metabolism
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Immunohistochemistry
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In Vitro Techniques
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Intercellular Signaling Peptides and Proteins / genetics
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Intercellular Signaling Peptides and Proteins / metabolism
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Interferon Regulatory Factor-1 / genetics
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Interferon Regulatory Factor-1 / metabolism
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Male
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Neoplasm Proteins / genetics
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Neoplasm Proteins / metabolism
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Phosphorylation
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RNA, Messenger / metabolism
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Rats
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Rats, Sprague-Dawley
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Reproducibility of Results
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Reverse Transcriptase Polymerase Chain Reaction
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Vasoconstriction / genetics*
Substances
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CCN2 protein, rat
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HSP27 Heat-Shock Proteins
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Heat-Shock Proteins
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Hspb1 protein, rat
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Immediate-Early Proteins
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Intercellular Signaling Peptides and Proteins
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Interferon Regulatory Factor-1
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Irf1 protein, rat
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Neoplasm Proteins
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RNA, Messenger
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Stip1 protein, rat
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Connective Tissue Growth Factor