The lipid soluble fraction of curcuma longa, i.e. herbal medicament (HM) was isolated from rhizome of curcuma longa by solvent extraction method. The identification of chemical constituent present in HM was done with GC, GC-MS. The standardization of HM was done using HPLC method on the basis of three-marker compound isolated, i.e. ar-turmerone, turmerone and curlone. The effect of temperature, pH and light on stability of marker compounds of HM was studied. The composition of marker compound in HM was found to be 50-60%. The content of curcumnoids, another bioactive constituent present in HM was found to be 0.32-0.55%. The HM was found to be stable at different temperature and pH but light sensitive.