Abstract
Stable isotope labeling of amino acids in cell culture was used for Bifidobacterium longum. A comprehensive proteomic strategy was developed and validated by designing an appropriate semidefined medium that allows stable replacement of natural leucine by [(13)C6]leucine. Using this strategy, proteins having variations of at least 50% in their expression rates can be quantified with great confidence.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / chemistry
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Bacterial Proteins / metabolism
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Bacteriological Techniques
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Bifidobacterium / growth & development*
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Bifidobacterium / metabolism*
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Carbon Isotopes / chemistry
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Carbon Isotopes / metabolism
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Culture Media
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Isotope Labeling / methods*
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Leucine / metabolism*
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Molecular Sequence Data
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Peptides / chemistry
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Peptides / metabolism
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Proteome
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Proteomics / methods*
Substances
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Bacterial Proteins
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Carbon Isotopes
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Culture Media
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Peptides
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Proteome
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Leucine