LambdaN-GFP: an RNA reporter system for live-cell imaging

Nat Methods. 2007 Aug;4(8):633-6. doi: 10.1038/nmeth1065. Epub 2007 Jul 1.

Abstract

We describe a GFP-based RNA reporter system (lambdaN-GFP) to visualize RNA molecules in live mammalian cells. It consists of GFP fused to an arginine-rich peptide derived from the phage lambda N protein, lambdaN22, which binds a unique minimal RNA motif and can be used to tag any RNA molecule. LambdaN-GFP uses a small and easy to engineer RNA tag, reducing the likelihood of perturbing the function of the tagged RNA molecule.

MeSH terms

  • Animals
  • Genes, Reporter*
  • Green Fluorescent Proteins / genetics*
  • RNA / genetics*
  • Subcellular Fractions / metabolism

Substances

  • Green Fluorescent Proteins
  • RNA