Adaptation of the base-paired double-helix molecular architecture to extreme pressure

Nucleic Acids Res. 2007;35(14):4800-8. doi: 10.1093/nar/gkm511. Epub 2007 Jul 7.

Abstract

The behaviour of the d(GGTATACC) oligonucleotide has been investigated by X-ray crystallography at 295 K in the range from ambient pressure to 2 GPa (approximately 20,000 atm). Four 3D-structures of the A-DNA form (at ambient pressure, 0.55, 1.09 and 1.39 GPa) were refined at 1.60 or 1.65 A resolution. In addition to the diffraction pattern of the A-form, the broad meridional streaks previously explained by occluded B-DNA octamers within the channels of the crystalline A-form matrix were observed up to at least 2 GPa. This work highlights an important property of nucleic acids, their capability to withstand very high pressures, while keeping in such conditions a nearly invariant geometry of base pairs that store and carry genetic information. The double-helix base-paired architecture behaves as a molecular spring, which makes it especially adapted to very harsh conditions. These features may have contributed to the emergence of a RNA World at prebiotic stage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Pairing
  • Crystallography, X-Ray
  • DNA / chemistry*
  • DNA, A-Form / chemistry*
  • Hydrostatic Pressure
  • Models, Molecular*
  • Nucleic Acid Conformation
  • Oligodeoxyribonucleotides / chemistry*

Substances

  • DNA, A-Form
  • Oligodeoxyribonucleotides
  • d(GGTATACC)
  • DNA

Associated data

  • PDB/2PKV
  • PDB/2PL4
  • PDB/2PL8
  • PDB/2PLB