Abstract
Two anti-mycobacterial peptides with a lasso structure, named lariatins A and B, were separated by HP-20 and ODS column chromatographies and purified by HPLC from the culture broth of Rhodococcus jostii K01-B0171, which was isolated from soil aggregates collected in Yunnan, China. Lariains A and B showed growth inhibition against Mycobacterium smegmatis with MIC values of 3.13 and 6.25 microg/ml in agar dilution method, respectively. Furthermore, lariatin A inhibited the growth of Mycobacterium tuberculosis with an MIC of 0.39 microg/ml in liquid microdilution method.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Antitubercular Agents / chemistry
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Antitubercular Agents / isolation & purification*
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Antitubercular Agents / metabolism
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Antitubercular Agents / pharmacology*
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China
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Fermentation
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Mycobacterium / drug effects
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Peptides, Cyclic / chemistry
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Peptides, Cyclic / isolation & purification*
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Peptides, Cyclic / metabolism
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Peptides, Cyclic / pharmacology*
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Phylogeny
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Polymerase Chain Reaction
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Rhodococcus / chemistry*
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Rhodococcus / classification
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Rhodococcus / isolation & purification
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Rhodococcus / metabolism
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Soil Microbiology
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Spectrometry, Mass, Fast Atom Bombardment
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Spectrophotometry, Infrared
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Spectrophotometry, Ultraviolet
Substances
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Antitubercular Agents
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Peptides, Cyclic
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lariatin A
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lariatin B