Abstract
Solid-state culture encourages high-level enzyme secretion by Aspergillus oryzae. Using the real-time quantitative reverse transcriptase-polymerase chain reaction, we confirmed that expression of the glucoamylase-encoding gene in A. oryzae cultured in solid-state culture depends on the water content of the culture.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Aspergillus oryzae / enzymology
-
Aspergillus oryzae / genetics
-
Culture Media*
-
Gene Expression / physiology*
-
Glucan 1,4-alpha-Glucosidase / biosynthesis
-
Glucan 1,4-alpha-Glucosidase / genetics*
-
Water*
Substances
-
Culture Media
-
Water
-
Glucan 1,4-alpha-Glucosidase