Molecular diagnosis of Kingella kingae pericarditis by amplification and sequencing of the 16S rRNA gene

J Clin Microbiol. 2007 Sep;45(9):3133-4. doi: 10.1128/JCM.00809-07. Epub 2007 Jul 18.

Abstract

Kingella kingae is a fastidious gram-negative bacillus that is considered an emerging pathogen in pediatric settings but remains less common in adults. Here we describe a case of pericarditis in an immunocompetent adult host. The microorganism was identified directly from the clinical sample by molecular techniques, i.e., 16S rRNA gene amplification and sequencing.

Publication types

  • Case Reports

MeSH terms

  • Adult
  • Anti-Bacterial Agents / therapeutic use
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Female
  • Humans
  • Kingella kingae / genetics
  • Kingella kingae / isolation & purification*
  • Neisseriaceae Infections / drug therapy
  • Neisseriaceae Infections / microbiology*
  • Nucleic Acid Amplification Techniques
  • Pericarditis / drug therapy
  • Pericarditis / microbiology*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA

Substances

  • Anti-Bacterial Agents
  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S