Abstract
Inhibitory GABAergic interneurons of the mouse neocortex are a highly heterogeneous population of neurons that originate from the ventral telencephalon and migrate tangentially up into the developing cortical plate. The majority of cortical interneurons arise from a transient embryonic structure known as the medial ganglionic eminence (MGE), but how the remarkable diversity is specified in this region is not known. We have taken a genetic fate mapping strategy to elucidate the temporal origins of cortical interneuron subtypes within the MGE. We used an inducible form of Cre under the regulation of Olig2, a basic helix-loop-helix transcription factor highly expressed in neural progenitors of the MGE. We observe that the physiological subtypes of cortical interneurons are, to a large degree, unique to their time point of generation.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Action Potentials / physiology
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Age Factors
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Animals
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Animals, Newborn
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Basic Helix-Loop-Helix Transcription Factors / genetics
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Basic Helix-Loop-Helix Transcription Factors / metabolism*
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Body Patterning / physiology
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Cell Differentiation / genetics
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Cell Differentiation / physiology
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Cell Movement / genetics
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Cell Movement / physiology*
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Cerebral Cortex / cytology*
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Cerebral Cortex / embryology*
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Embryo, Mammalian
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Gene Expression Regulation, Developmental / physiology
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Green Fluorescent Proteins / metabolism
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In Vitro Techniques
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Interneurons / classification
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Interneurons / physiology*
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Mice
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Mice, Transgenic
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism*
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Oligodendrocyte Transcription Factor 2
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Patch-Clamp Techniques / methods
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Stem Cells / physiology*
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Telencephalon / cytology
Substances
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Basic Helix-Loop-Helix Transcription Factors
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Nerve Tissue Proteins
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Olig2 protein, mouse
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Oligodendrocyte Transcription Factor 2
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enhanced green fluorescent protein
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Green Fluorescent Proteins