Abstract
Sialyl Tn (STn) and sialyl lactoside derivatives containing O-acetylated sialic acid residues have been chemoenzymatically synthesized using a one-pot three-enzyme system and conjugated to biotinylated human serum albumin (HSA) using an adipic acid para-nitrophenyl ester coupling reagent. This approach provides an efficient and general protocol for preparing carbohydrate-protein conjugates containing base-sensitive groups.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylation
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Biotin / chemistry*
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Glycosides / chemistry*
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Humans
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Molecular Structure
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Serum Albumin / chemistry*
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Serum Albumin / metabolism*
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Sialic Acids / chemical synthesis*
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Sialic Acids / chemistry
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Sialic Acids / metabolism*
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Sialyltransferases / metabolism*
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Substances
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Glycosides
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Serum Albumin
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Sialic Acids
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Biotin
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Sialyltransferases