Three new lanostanoid triterpenes named 3beta,7beta-di- hydroxy-11,15,23-trioxo-lanost-8,16-dien-26-oic acid (1), 3beta,7beta-dihydroxy-11,15,23-trioxo-lanost-8,16-dien-26-oic acid methyl ester (2), and 12beta-acetoxy-3beta,7beta-dihydroxy-11,15,23-trioxo-lanost-8,16-dien-26-oic acid (3) were isolated from the fruit bodies of Ganoderma lucidum. They all show a Delta(16, 17) double bond unprecedented in such types of lanostanoid triterpenes possessing the side chain at C-17. The complete NMR assignments for these compounds were carried out using (1)H, (13)C, DEPT, COSY, HSQC, HMBC, and ROESY NMR experiments.