Abstract
DAF-2, an insulin receptor-like protein, regulates metabolism, development, and aging in Caenorhabditis elegans. In a quantitative proteomic study, we identified 86 proteins that were more or less abundant in long-lived daf-2 mutant worms than in wild-type worms. Genetic studies on a subset of these proteins indicated that they act in one or more processes regulated by DAF-2, including entry into the dauer developmental stage and aging. In particular, we discovered a compensatory mechanism activated in response to reduced DAF-2 signaling, which involves the protein phosphatase calcineurin.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Aging / physiology
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Animals
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Caenorhabditis elegans / genetics
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Caenorhabditis elegans / metabolism*
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Caenorhabditis elegans / physiology
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Caenorhabditis elegans Proteins / genetics
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Caenorhabditis elegans Proteins / metabolism*
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Calcineurin / metabolism
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Carbohydrate Metabolism
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Gene Expression Regulation
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Genes, Helminth
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Longevity / physiology
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Mass Spectrometry / methods
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Models, Animal
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Mutation
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Proteomics
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RNA Interference
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Receptor, Insulin / genetics
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Receptor, Insulin / metabolism*
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Signal Transduction*
Substances
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Caenorhabditis elegans Proteins
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DAF-2 protein, C elegans
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Receptor, Insulin
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Calcineurin