Fourier-domain digital holographic optical coherence imaging of living tissue

Appl Opt. 2007 Aug 1;46(22):4999-5008. doi: 10.1364/ao.46.004999.

Abstract

Digital holographic optical coherence imaging is a full-frame coherence-gated imaging approach that uses a CCD camera to record and reconstruct digital holograms from living tissue. Recording digital holograms at the optical Fourier plane has advantages for diffuse targets compared with Fresnel off-axis digital holography. A digital hologram captured at the Fourier plane requires only a 2D fast Fourier transform for numerical reconstruction. We have applied this technique for the depth-resolved imaging of rat osteogenic tumor multicellular spheroids and acquired cross-section images of the anterior segment and the retinal region of a mouse eye. A penetration depth of 1.4 mm for the tumor spheroids was achieved.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Algorithms
  • Animals
  • Equipment Design
  • Eye / pathology*
  • Fourier Analysis
  • Holography / instrumentation*
  • Holography / methods
  • Image Interpretation, Computer-Assisted
  • Image Processing, Computer-Assisted / instrumentation
  • Image Processing, Computer-Assisted / methods*
  • Mice
  • Microscopy, Video / instrumentation
  • Microscopy, Video / methods*
  • Models, Statistical
  • Optics and Photonics*
  • Rats
  • Retina / pathology*
  • Spheroids, Cellular / pathology
  • Tumor Cells, Cultured