Capturing the interaction potential of amyloidogenic proteins

Phys Rev Lett. 2007 Jul 13;99(2):028101. doi: 10.1103/PhysRevLett.99.028101. Epub 2007 Jul 13.

Abstract

Experimentally derived static structure factors obtained for the aggregation-prone protein insulin were analyzed with a statistical mechanical model based on the Derjaguin-Landau-Verwey-Overbeek potential. The data reveal that the protein self-assembles into equilibrium clusters already at low concentrations. Furthermore, striking differences regarding interaction forces between aggregation-prone proteins such as insulin in the preaggregated regime and natively stable globular proteins are found.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amyloid / chemistry*
  • Biophysics / methods*
  • Humans
  • Hydrogen-Ion Concentration
  • Insulin / chemistry
  • Insulin / metabolism*
  • Models, Statistical
  • Models, Theoretical
  • Muramidase / chemistry
  • Peptides / chemistry*
  • Probability
  • Protein Binding
  • Protein Denaturation
  • Scattering, Radiation
  • Static Electricity
  • Temperature

Substances

  • Amyloid
  • Insulin
  • Peptides
  • Muramidase