Abstract
A phytochemical investigation of the EtOH extract of Hoveniae Lignum yielded four phenolic compounds, phloretin (1), 5-(4'-hydroxyphenyl)-gamma-valerolactone (2), (-)-epiafzelechin (3), and maesopsin (4). Compound 1 was hepatoprotective against tacrine-induced cytotoxicity in human liver-derived Hep G2 cells with an EC50 value of 37.55 +/- 0.42 microM. Compound 1 (0.4-200 microM) also significantly reduced tert-butyl hydroperoxide-induced cytotoxicity in rat primary hepatocytes as measured by the cellular leakage of lactate dehydrogenase and the level of aspartate transaminase.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antioxidants / pharmacology
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Aspartate Aminotransferases / metabolism
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Cell Line, Tumor
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Cell Survival / drug effects
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Cholinesterase Inhibitors / toxicity
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Hepatocytes / drug effects*
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Humans
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L-Lactate Dehydrogenase / metabolism
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Magnetic Resonance Spectroscopy
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Male
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Mass Spectrometry
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Phloretin / pharmacology
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Plant Extracts / chemistry
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Plant Extracts / pharmacology
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Rats
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Rats, Sprague-Dawley
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Rhamnaceae / chemistry*
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Spectrophotometry, Ultraviolet
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Tacrine / antagonists & inhibitors
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Tacrine / toxicity
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tert-Butylhydroperoxide / toxicity
Substances
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Antioxidants
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Cholinesterase Inhibitors
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Plant Extracts
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Tacrine
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tert-Butylhydroperoxide
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L-Lactate Dehydrogenase
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Aspartate Aminotransferases
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Phloretin