Tibet pig is a special Chinese indigenous pig breed, which could survive in high-altitude areas of Tibet. In the present study, the genotypes of ESR, FSHbeta, and PRLR gene, the three known major genes of reproductive traits, were identified in 202 Tibet pig individuals. The results showed that the predominant genotypes of ESR, FSHbeta, and PRLR gene were BB, AB and AA, respectively. The genotype frequencies of the three genes were in Hardy-Weinberg equilibrium. The frequencies of desired alleles of the three genes were higher than those of other alleles, which indicated that Tibet pig had a good potential for improving reproductive traits.