Abstract
An outbreak of contagious ecthyma in goats in central Taiwan was investigated. The disease was diagnosed by physical and histopathologic examinations, and the etiology of the disease was identified as orf virus by electron microscopy and polymerase chain reaction (PCR) and sequence of major envelope protein (B2L) gene. The entire protein-coding region of B2L gene were cloned and sequenced. Phylogenetic analysis of B2L amino acid sequences showed that the orf virus identified in this outbreak was closer to the Indian ORFV-Mukteswar 59/05 isolate. This is the first report on the molecular characterization of orf virus in Taiwan.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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DNA, Viral / chemistry
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DNA, Viral / genetics
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DNA, Viral / isolation & purification
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Disease Outbreaks*
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Ecthyma, Contagious / epidemiology*
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Ecthyma, Contagious / pathology
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Ecthyma, Contagious / physiopathology
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Ecthyma, Contagious / virology*
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Goats
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Microscopy, Electron, Transmission
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Molecular Sequence Data
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Orf virus / genetics
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Orf virus / isolation & purification*
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Orf virus / ultrastructure
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Phylogeny
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Polymerase Chain Reaction
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Taiwan / epidemiology
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Viral Envelope Proteins / genetics
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Virion / ultrastructure
Substances
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DNA, Viral
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Viral Envelope Proteins