Role of concanavalin A lectin in recognition of pterygium remnant after surgical excision: preliminary results of a prospective study

Indian J Ophthalmol. 2007 Sep-Oct;55(5):349-53. doi: 10.4103/0301-4738.33820.

Abstract

Background: Pterygium is one of the most common conjunctival diseases among ophthalmic pathologies. The frequency of recurrences is high, either after surgical treatment or after treatment combined with mitomycin C or beta-radiation therapy.

Aims: The purpose of this study was to determine whether concanavalin A (ConA) lectin bound to the pterygial surface can be used to detect recurrence or remnants of pterygium after surgical excision.

Materials and methods: This was a prospective study on 20 patients with pterygium, divided in five stages, pre-surgery, early post-surgery (24h), late post-surgery (seven days), very late post-surgery (four weeks) and two months after the procedure. A drop of fluorescein-marked Con A (35 microg/mL) was instilled in the lower conjunctival eyelid sac and the eye was exposed to the light of a Wood's lamp for an average of five seconds.

Results: Out of the 20 patients, eight patients were found to have fluorescent stretch marks over the scar corresponding to residual pterygial tissue at four weeks; two months after the procedure of re-surgery we observed no fluorescent remnants. All residual pterygia were confirmed through histochemistry studies.

Conclusion: It was possible to detect remnants of pterygium in postoperative patients and recurrences in early pre-clinical stages through the visualization of fluorescent ConA bound to the pterygial surface.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Animals
  • Concanavalin A*
  • Conjunctiva / pathology*
  • Conjunctiva / surgery
  • Diagnosis, Differential
  • Disease Models, Animal
  • Follow-Up Studies
  • Humans
  • Middle Aged
  • Mitogens*
  • Photomicrography
  • Postoperative Care / methods
  • Prospective Studies
  • Pterygium / diagnosis*
  • Pterygium / surgery
  • Rats
  • Recurrence
  • Reproducibility of Results

Substances

  • Mitogens
  • Concanavalin A