Abstract
Bone resorption by osteoclasts stimulates bone formation by osteoblasts. To isolate osteoblastic factors coupled with osteoclast activity, we performed microarray and cluster analysis of 8 tissues including bone, and found that among 10,490 genes, osteomodulin (OMD), an extracellular matrix keratan sulfate proteoglycan, was simultaneously induced with osteoclast-specific markers such as MMP9 and Acp5. OMD expression was detected in osteoblasts and upregulated during osteoblast maturation. OMD expression in osteoblasts was also detected immunohistochemically using a specific antibody against OMD. The immunoreactivity against OMD decreased in op/op mice, which lack functional macrophage colony stimulating factor (M-CSF) and are therefore defective in osteoclast formation, when compared to wild-type littermates. OMD expression in op/op mice was upregulated by M-CSF treatment. Since the M-CSF receptor c-Fms was not expressed in osteoblasts, it is likely that OMD is an osteoblast maturation marker that is induced by osteoclast activity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells
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Acid Phosphatase / genetics
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Animals
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Cell Line
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Cells, Cultured
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Extracellular Matrix Proteins / genetics*
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Extracellular Matrix Proteins / metabolism
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Fluorescent Antibody Technique
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Gene Expression / drug effects
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Gene Expression Profiling*
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Humans
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Isoenzymes / genetics
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Macrophage Colony-Stimulating Factor / pharmacology
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Male
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Matrix Metalloproteinase 9 / genetics
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Mice
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Mice, Mutant Strains
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Oligonucleotide Array Sequence Analysis / methods
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Osteoblasts / cytology
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Osteoblasts / drug effects
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Osteoblasts / metabolism*
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Osteoclasts / cytology
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Osteoclasts / drug effects
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Osteoclasts / metabolism*
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Osteopetrosis / genetics
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Osteopetrosis / metabolism
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Osteopetrosis / pathology
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Proteoglycans / genetics*
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Proteoglycans / metabolism
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Rats
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Rats, Wistar
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Reverse Transcriptase Polymerase Chain Reaction
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Tartrate-Resistant Acid Phosphatase
Substances
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Extracellular Matrix Proteins
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Isoenzymes
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Proteoglycans
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osteoadherin
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Macrophage Colony-Stimulating Factor
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ACP5 protein, human
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Acid Phosphatase
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Acp5 protein, mouse
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Acp5 protein, rat
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Tartrate-Resistant Acid Phosphatase
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Matrix Metalloproteinase 9