A single nucleotide polymorphism in the DNA polymerase gamma gene of Saccharomyces cerevisiae laboratory strains is responsible for increased mitochondrial DNA mutability

Genetics. 2007 Oct;177(2):1227-31. doi: 10.1534/genetics.107.079293. Epub 2007 Aug 24.

Abstract

In the Saccharomyces cerevisiae strains used for genome sequencing and functional analysis, the mitochondrial DNA replicase Mip1p contains a single nucleotide polymorphism changing the strictly conserved threonine 661 to alanine. This substitution is responsible for the increased rate of mitochondrial DNA point mutations and deletions in these strains.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Substitution
  • DNA Polymerase gamma
  • DNA, Mitochondrial / genetics*
  • DNA-Directed DNA Polymerase / genetics*
  • Genes, Fungal
  • Kinetics
  • Mutation*
  • Point Mutation
  • Polymorphism, Single Nucleotide*
  • Saccharomyces cerevisiae Proteins / genetics*
  • Sequence Deletion

Substances

  • DNA, Mitochondrial
  • Saccharomyces cerevisiae Proteins
  • DNA Polymerase gamma
  • DNA-Directed DNA Polymerase