Sodium-23 and lithium-7 NMR spin-lattice relaxation measurements in the study intercalation in DNA

Biophys Chem. 1991 Nov;41(2):113-24. doi: 10.1016/0301-4622(91)80011-f.

Abstract

Sodium-23 spin-lattice relaxation rate (the reciprocal relaxation time) measurements have been used to study the intercalation of 9-aminoacridine in calf thymus DNA. The results are analyzed by a two state model based on the counterion condensation theory and a theory for the quadrupolar relaxation of counterions in polyelectrolyte solutions. It is shown that change of the solvent from H2O to D2O has a negligible effect on the intercalation process. Furthermore, an attempt is made to analyze the dependence of the 7Li spin-lattice relation rate on intercalation of 9-aminoacridine in LiDNA. It is shown that both quadrupolar and dipolar mechanisms contribute to the bound 7Li relation rate, and that both these contributions are reduced upon intercalation of 9-aminoacridine.

MeSH terms

  • Aminacrine / chemistry
  • Animals
  • Cattle
  • DNA / chemistry*
  • Intercalating Agents / chemistry*
  • Lithium
  • Magnetic Resonance Spectroscopy
  • Sodium Isotopes
  • Thymus Gland / chemistry

Substances

  • Intercalating Agents
  • Sodium Isotopes
  • Aminacrine
  • DNA
  • Lithium