The induction of microsomal NADPH:cytochrome P450 and NADH:cytochrome b(5) reductases by long-term salt treatment of cotton (Gossypium hirsutum L.) and bean (Phaseolus vulgaris L.) plants

Plant Physiol Biochem. 2007 Sep;45(9):691-5. doi: 10.1016/j.plaphy.2007.07.005. Epub 2007 Jul 22.

Abstract

We studied the effect of salinity on the activity of microsomal NADPH:cytochrome P450 reductase (CPR, EC 1.6.2.4) and NADH:ferricytochrome b(5) oxidoreductase (B5R, EC 1.6.2.2) in two dicotyledonous plant species differing in their sensitivity to salt, cotton (Gossypium hirsutum L. cv Ogosta) and common bean (Phaseolus vulgaris L. cv Dobrujanski 7). A significant inhibition of fresh weight of salt-treated bean plants was observed, while cotton was affected to a much lesser degree. NaCl application resulted in a significant increase in the activity of both reductases, but was more pronounced in salt-tolerant cotton. We suppose that alterations in B5R and CPR activities may be targeted to the maintenance of membrane lipids. Most probably, plants use both enzymes (B5R and CPR) and their respective electron donors (NADH and NADPH) to reduce cytochrome b(5), which can donate reducing equivalents to a series of lipid-modification reactions such as desaturation and hydroxylation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cytochrome-B(5) Reductase / genetics
  • Cytochrome-B(5) Reductase / metabolism*
  • Dose-Response Relationship, Drug
  • Gene Expression Regulation, Plant / drug effects
  • Gossypium / drug effects
  • Gossypium / enzymology*
  • Gossypium / genetics
  • Microsomes / enzymology*
  • NADPH-Ferrihemoprotein Reductase / genetics
  • NADPH-Ferrihemoprotein Reductase / metabolism*
  • Phaseolus / drug effects
  • Phaseolus / enzymology*
  • Phaseolus / genetics
  • Plant Roots
  • Plant Shoots
  • Sodium Chloride / pharmacology*

Substances

  • Sodium Chloride
  • Cytochrome-B(5) Reductase
  • NADPH-Ferrihemoprotein Reductase