Abstract
Esterase A4 (EA4) is a timer protein found in diapause eggs of the silkworm Bombyx mori. The gene for this metalloglycoprotein was cloned from B. mori eggs and expressed using a baculovirus expression system in silkworm pupae. Crystals of the purified protein have been grown that diffract to beyond 2.1 A resolution at 100 K using synchrotron radiation. The protein crystals belong to space group P2(1), with unit-cell parameters a = 47.1, b = 73.9, c = 47.4 A, beta = 104.1 degrees. With one dimer per asymmetric unit, the crystal volume per unit protein weight (V(M)) is 2.3 A3 Da(-1) and the solvent content is 47%.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Baculoviridae
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Base Sequence
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Bombyx / enzymology
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Carboxylesterase / chemistry*
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Carboxylesterase / isolation & purification
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Crystallization
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Crystallography, X-Ray
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DNA Primers
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Female
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Glycoproteins / chemistry
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Glycoproteins / isolation & purification
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Glycosylation
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Metalloproteins / chemistry
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Metalloproteins / isolation & purification
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Molecular Sequence Data
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Ovum / enzymology
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Recombinant Proteins / chemistry
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Recombinant Proteins / isolation & purification
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Substances
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DNA Primers
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Glycoproteins
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Metalloproteins
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Recombinant Proteins
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Carboxylesterase