Conformational study of signal peptides of the LamB protein

Int J Biol Macromol. 1991 Aug;13(4):241-6. doi: 10.1016/0141-8130(91)90080-e.

Abstract

A molecular mechanics study of a portion of the signal peptide of LamB protein, a mutant and two revertants, has been carried out. The peptides studied are: (I) Leu-Pro-Leu-Ala-Val-Ala-Val-Ala-Ala-Gly-Val for the wild type signal peptide; a mutant which shows no export capability (II), Leu-Pro-Val-Ala-Ala-Gly-Val; and two revertants with replacements of Pro by Leu (III), and Gly by Cys (IV) respectively. The results found are in agreement with the experimental data available; the aim of this work being to provide evidence of conformational features necessary along the export mechanisms. The present study suggests that both an alpha helix formation capability and a certain hydrophobicity of the peptide chain are the characteristics required for export competence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Outer Membrane Proteins / chemistry
  • Models, Molecular
  • Molecular Sequence Data
  • Porins
  • Protein Conformation
  • Protein Sorting Signals / chemistry*
  • Receptors, Virus / chemistry*
  • Receptors, Virus / genetics
  • Thermodynamics

Substances

  • Bacterial Outer Membrane Proteins
  • Porins
  • Protein Sorting Signals
  • Receptors, Virus
  • maltoporins