Extension of the range of recognition sequences for triple helix formation by oligonucleotides containing guanines and thymines

C R Acad Sci III. 1991;313(13):585-90.

Abstract

Oligodeoxynucleotides containing G and T can bind to homopurine.homopyrimidine sequences on double-stranded DNA by forming C.G x G and T.A x T base triplets. The orientation of the third strand in such triple helices depends on the number of GpT and TpG steps. Therefore a single oligonucleotide can be designed to bind to two consecutive homopurine.homopyrimidine sequences where the two homopurine stretches alternate on the two strands of DNA. The oligonucleotide switches from one homopurine strand to the other at the junction between the two sequences. This result shows that it is possible to extend the range of DNA sequences that can be recognized by a single oligonucleotide.

MeSH terms

  • Base Sequence
  • DNA / chemistry*
  • DNA / genetics
  • Guanosine / chemistry
  • Humans
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Thymidine / chemistry

Substances

  • Guanosine
  • DNA
  • Thymidine