Solid-phase synthesis and thermal denaturation study of cyclic PNAs targeting the HIV-1 TAR RNA loop

Gregory Upert; Mohamed Mehiri; Audrey Di Giorgio; Roger Condom; Nadia Patino

doi:10.1016/j.bmcl.2007.07.030

Solid-phase synthesis and thermal denaturation study of cyclic PNAs targeting the HIV-1 TAR RNA loop

Bioorg Med Chem Lett. 2007 Nov 1;17(21):6026-30. doi: 10.1016/j.bmcl.2007.07.030. Epub 2007 Jul 21.

Authors

Gregory Upert¹, Mohamed Mehiri, Audrey Di Giorgio, Roger Condom, Nadia Patino

Affiliation

¹ Laboratoire de Chimie des Molécules Bioactives et des Arômes (LCMBA), UMR UNSA-CNRS 6001, Université de Nice-Sophia Antipolis, 06108 Nice Cedex 2, France.

PMID: 17826994
DOI: 10.1016/j.bmcl.2007.07.030

Abstract

Cyclic PNAs targeting the HIV-1 TAR RNA loop have been synthesized following a convenient solid-phase strategy which allows on-resin cyclisation. UV-monitored thermal denaturation studies demonstrate that these cyclic PNAs are able to strongly interact with their TAR RNA target, very likely through the formation of a six-base pair stable complex, involving the TAR RNA loop.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

HIV-1 / genetics*
Peptide Nucleic Acids / chemical synthesis*
Peptide Nucleic Acids / chemistry
Peptide Nucleic Acids / pharmacology
Peptides, Cyclic / chemical synthesis*
Peptides, Cyclic / chemistry
Peptides, Cyclic / pharmacology
RNA, Viral / drug effects*
Spectrophotometry, Ultraviolet

Substances

Peptide Nucleic Acids
Peptides, Cyclic
RNA, Viral