The metabolism of Zn and tissue mineral concentrations were studied after a single oral 65Zn dose in 10 6-wk-old Holstein calves injected subcutaneously daily with 0 (control) or 10 mg of sometribove (recombinant methionyl bST) for 6 wk. Zinc-65 absorption was not significantly affected by bST; its concentration in the semitendinosus muscle was reduced by 32% in the bST calves, but concentrations in liver, pancreas, spleen, kidney, heart, small intestine, testicle, and rib were not different from controls. Manganese content was reduced by 27% in liver, 60% in kidney, 99% in spleen, 92% in testicles, and 33% in rib. Iron content of pancreas, spleen, and testicle and Zn content of rib were increased in the bST calves. The data indicate that Zn metabolism was not affected adversely by bST. Manganese content of several tissues was significantly reduced in the bST calves; however, no clinical signs of an Mn deficiency were evident.