Speed-mapping quantitative trait loci using microarrays

Nat Methods. 2007 Oct;4(10):839-41. doi: 10.1038/nmeth1084. Epub 2007 Sep 16.

Abstract

We developed a rapid, economical method for high-resolution quantitative trait locus (QTL) mapping using microarrays for selective genotyping of pooled DNA samples. We generated 21,207 F2 flies from two inbred Drosophila melanogaster strains with known QTLs affecting lifespan, and hybridized DNA pools of young and old flies to microarrays. We used changes of gene frequency of 2,326 single-feature polymorphisms (SFPs) to map previously identified and additional QTLs affecting lifespan.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Drosophila melanogaster / genetics
  • Drosophila melanogaster / growth & development
  • Female
  • Gene Expression Profiling / methods*
  • Longevity / genetics
  • Male
  • Oligonucleotide Array Sequence Analysis / methods*
  • Polymorphism, Genetic / genetics
  • Quantitative Trait Loci*