Mutations in preS genes of genotype C hepatitis B virus in patients with chronic hepatitis B and hepatocellular carcinoma

J Gastroenterol. 2007 Sep;42(9):761-8. doi: 10.1007/s00535-007-2085-1. Epub 2007 Sep 25.

Abstract

Background: Hepatitis B virus (HBV) preS mutations are frequently isolated from patients with severe forms of liver disease. Meanwhile, genotype C has been shown to cause more serious liver disease than genotype B. This study assesses the frequency of preS mutation in Chinese patients with genotype C chronic HBV infection and its relation to liver damage.

Methods: Seventy-nine persistently infected patients (25 asymptomatic carriers, 28 with chronic hepatitis, and 26 with hepatocellular carcinoma) with genotype C HBV were analyzed. Levels of HBV DNA, hepatitis B e antigen (HBeAg), alanine aminotransferase, and aspartate transaminase and mutations in the preS region were determined.

Results: The correlations of preS deletion with disease progression were distinct: preS deletion mutations were more commonly found in the hepatocellular carcinoma (HCC) group than in the chronic hepatitis B (CHB) or asymptomatic carrier (ASC) groups, with the frequencies of 38.46% (10/26) in the HCC, 7.14% (2/28) in the CHB, and 4.00% (1/25) in the ASC (P = 0.001) groups. The HBeAg-positive rate and HBV DNA levels were comparable between patients with the preS mutation and those without.

Conclusions: PreS deletion mutations of genotype C HBV might play a role in HBV-related hepatocarcinogenesis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Carcinoma, Hepatocellular / blood
  • Carcinoma, Hepatocellular / etiology
  • Carcinoma, Hepatocellular / genetics*
  • DNA, Viral / genetics*
  • Female
  • Genotype
  • Hepatitis B Surface Antigens / genetics*
  • Hepatitis B e Antigens / blood
  • Hepatitis B virus / genetics*
  • Hepatitis B virus / immunology
  • Hepatitis B, Chronic / blood
  • Hepatitis B, Chronic / complications
  • Hepatitis B, Chronic / genetics*
  • Humans
  • Immunoenzyme Techniques
  • Liver Neoplasms / blood
  • Liver Neoplasms / etiology
  • Liver Neoplasms / genetics*
  • Male
  • Middle Aged
  • Mutation*
  • Polymerase Chain Reaction
  • Protein Precursors / genetics*
  • Risk Factors
  • Viral Envelope Proteins

Substances

  • DNA, Viral
  • Hepatitis B Surface Antigens
  • Hepatitis B e Antigens
  • Protein Precursors
  • Viral Envelope Proteins
  • presurface protein 1, hepatitis B surface antigen