Vaccination against Ostertagia ostertagi with subfractions of the protective ES-thiol fraction

Vet Parasitol. 2007 Nov 10;149(3-4):239-45. doi: 10.1016/j.vetpar.2007.08.014. Epub 2007 Sep 18.

Abstract

Previous vaccination trials against Ostertagia ostertagi in cattle have demonstrated the protective capacity of a protein fraction termed ES-thiol, which is enriched for activation-associated secreted proteins (ASPs) and cysteine proteases. In this study, ES-thiol was subfractionated through Q-Sepharose anion exchange chromatography to determine whether the ASPs and/or the cysteine proteases are responsible for the induced protection. Calves (seven/group) were immunized three times intramuscularly with 100 microg of ES-thiol or equivalent amounts of an ASP-enriched fraction, a cysteine protease-enriched fraction or a rest fraction, with QuilA adjuvant. A negative control group only received QuilA. After the final immunization the animals were challenged with a trickle infection of 25,000 infectious L3 larvae (1000 L3/day; 5 days/week). During a 2-month period the geometric mean cumulative faecal egg count (FEC) of the ES-thiol group was reduced by 62% compared to the QuilA control group (P<0.05). Groups injected with the ASP-enriched, the cysteine protease-enriched and the rest fraction demonstrated a reduction in cumulative FEC of 74, 80 and 70%, respectively (P<0.01). Although no significant reductions in worm burdens were observed, adult male and female worms were significantly smaller in all vaccinated groups (P<0.05), except for male worms from the ES-thiol group. These results suggest the protective capacity of ASPs and the presence of other protective antigens in the ES-thiol fraction.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Helminth / blood
  • Antigens, Helminth / immunology*
  • Cattle
  • Cattle Diseases / parasitology
  • Cattle Diseases / prevention & control*
  • Feces / parasitology
  • Female
  • Helminth Proteins / immunology*
  • Larva
  • Ostertagia / immunology*
  • Ostertagiasis / immunology
  • Ostertagiasis / prevention & control
  • Ostertagiasis / veterinary*
  • Time Factors
  • Vaccination / veterinary*
  • Vaccines / immunology*

Substances

  • Antibodies, Helminth
  • Antigens, Helminth
  • Helminth Proteins
  • Vaccines
  • excretory-secretory protein, Ostertagia