Structural analysis of the rat uricase gene and evidence that lysine 164 is involved in the substrate-binding site of the enzyme

MeSH terms

• Amino Acid Sequence
• Animals
• Base Sequence
• Binding Sites
• Cloning, Molecular / methods
• Cosmids
• Escherichia coli / genetics
• Genomic Library
• Kinetics
• Lysine*
• Molecular Sequence Data
• Mutagenesis, Site-Directed*
• Oligodeoxyribonucleotides
• Rats
• Recombinant Proteins / isolation & purification
• Recombinant Proteins / metabolism
• Restriction Mapping
• Urate Oxidase / genetics*
• Urate Oxidase / isolation & purification
• Urate Oxidase / metabolism
• Xanthine
• Xanthines / metabolism