Identification of enterohemorrhagic Escherichia coli O157-Specific DNA sequence obtained from amplified fragment length polymorphism analysis

Akihiko Tokunaga; Masanori Kawano; Masatoshi Okura; Sunao Iyoda; Haruo Watanabe; Ro Osawa

doi:10.1111/j.1348-0421.2007.tb03970.x

Identification of enterohemorrhagic Escherichia coli O157-Specific DNA sequence obtained from amplified fragment length polymorphism analysis

Microbiol Immunol. 2007;51(9):883-8. doi: 10.1111/j.1348-0421.2007.tb03970.x.

Authors

Akihiko Tokunaga¹, Masanori Kawano, Masatoshi Okura, Sunao Iyoda, Haruo Watanabe, Ro Osawa

Affiliation

¹ Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan.

PMID: 17895605
DOI: 10.1111/j.1348-0421.2007.tb03970.x

Abstract

An approximately 1.1 kbp fragment that was commonly observed only in the enterohemorrhagic Escherichia coli (EHEC) O157 strains in an analysis of amplified fragment length polymorphism was found to be a partial gene sequence encoding the locus of toxB and a useful molecular marker for the identification of EHEC O157.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amplified Fragment Length Polymorphism Analysis*
Animals
Base Sequence
DNA, Bacterial / chemistry
DNA, Bacterial / genetics*
Escherichia coli Infections / microbiology
Escherichia coli O157 / classification
Escherichia coli O157 / genetics*
Escherichia coli Proteins / chemistry
Escherichia coli Proteins / genetics*
Escherichia coli Proteins / metabolism
Food Microbiology
Humans
Molecular Sequence Data
Sewage / microbiology

Substances

DNA, Bacterial
Escherichia coli Proteins
Sewage
toxB protein, E coli