[Application of MiniFiler kit in forensic DNA testing of low copy number template]

Fa Yi Xue Za Zhi. 2007 Aug;23(4):304-6.
[Article in Chinese]

Abstract

Objective: To detect low copy number of DNA samples by using a newly launched commercial miniSTR detection kit (MiniFiler) in forensic practice.

Methods: Low concentration and/or challenged forensic DNA samples were analyzed according to protocols provided by the manufacturer (Applied Biosystems, Foster City, USA).

Results: DNA samples as low as 10 pg could be amplified by MiniFiler kit, and the optimal DNA quantity was 40 pg or above.

Conclusion: MiniFiler kit can be used for analysis of low copy number STR.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • DNA Fingerprinting / instrumentation*
  • DNA Fingerprinting / methods
  • Forensic Genetics / methods*
  • Genotype
  • Humans
  • Polymerase Chain Reaction / instrumentation*
  • Polymerase Chain Reaction / methods
  • Reagent Kits, Diagnostic
  • Sensitivity and Specificity
  • Tandem Repeat Sequences
  • Templates, Genetic

Substances

  • Reagent Kits, Diagnostic