Abstract
The glucosinolates sinalbin and glucoraphanin were purified by strong ion-exchange displacement centrifugal partition chromatography (SIXCPC). The optimized conditions involved the biphasic solvent system ethyl acetate/n-butanol/water (3:2:5, v/v), the lipophilic anion-exchanger Aliquat 336 (trioctylmethylammonium chloride, 160 and 408 mM) and a sodium iodide solution (80 and 272 mM) as displacer. Amounts as high as 2.4 g of sinalbin and 2.6g of glucoraphanin were obtained in one step in 2.5 and 3.5h respectively, starting from 12 and 25 g of mustard and broccoli seed aqueous extracts, using a laboratory scale CPC column (200 mL inner volume).
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Centrifugation / methods*
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Chemical Fractionation / methods
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Choline / analogs & derivatives*
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Choline / isolation & purification
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Chromatography, Ion Exchange / methods*
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Glucosinolates / isolation & purification*
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Hydrogen-Ion Concentration
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Imidoesters / isolation & purification*
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Molecular Structure
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Mustard Plant / chemistry
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Oximes
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Plant Extracts / chemistry*
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Quaternary Ammonium Compounds / chemistry
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Seeds / chemistry
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Solvents / chemistry
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Sulfoxides
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Water / chemistry
Substances
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Glucosinolates
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Imidoesters
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Oximes
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Plant Extracts
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Quaternary Ammonium Compounds
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Solvents
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Sulfoxides
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Water
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trioctylmethylammonium
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Choline
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glucoraphanin
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sinalbin