Expression of angiopoietins and vascular endothelial growth factors and their clinical significance in acute myeloid leukemia

Hsin-An Hou; Wen-Chien Chou; Liang-In Lin; Jih-Luh Tang; Mei-Hsuan Tseng; Chi-Fei Huang; Ming Yao; Chien-Yuan Chen; Woei Tsay; Hwei-Fang Tien

doi:10.1016/j.leukres.2007.08.010

Expression of angiopoietins and vascular endothelial growth factors and their clinical significance in acute myeloid leukemia

Leuk Res. 2008 Jun;32(6):904-12. doi: 10.1016/j.leukres.2007.08.010. Epub 2007 Sep 29.

Authors

Hsin-An Hou¹, Wen-Chien Chou, Liang-In Lin, Jih-Luh Tang, Mei-Hsuan Tseng, Chi-Fei Huang, Ming Yao, Chien-Yuan Chen, Woei Tsay, Hwei-Fang Tien

Affiliation

¹ Division of Hematology, Department of Internal Medicine, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan.

PMID: 17904634
DOI: 10.1016/j.leukres.2007.08.010

Abstract

Angiogenic factors play an essential role in normal and pathologic angiogenesis, but their clinical role in acute myeloid leukemia (AML) remains unclear. We investigated the expression of Ang-1, Ang-2, Tie2, VEGF-A, and VEGF-C genes in bone marrow (BM) mononuclear cells by real-time quantitative PCR (RQ-PCR) in a cohort of 126 patients with newly diagnosed de novo AML and normal marrow donors. Here we show that high pre-treatment levels of Ang-2 in the BM indicate an unfavorable prognosis in AML. Only karyotype (hazard ratio 2.19, 95% CI 1.25-3.42, P=0.005) and expression of Ang-2 (hazard ratio 2.05, 95% CI 1.20-3.52, P=0.009), but not other angiogenic factors, were independent prognostic factors for overall survival by multivariate analysis. The prognostic significance of Ang-2 expression was more obvious in the subgroup of patients with intermediate-risk cytogenetics. Subgroup analysis showed that Ang-2 expression had prognostic impact on patients with low (but not high) Ang-1 or Tie2 levels, and on patients with high (but not low) VEGF-A or VEGF-C levels.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Adult
Aged
Aged, 80 and over
Angiopoietin-1 / genetics*
Angiopoietin-1 / metabolism
Angiopoietin-2 / genetics*
Angiopoietin-2 / metabolism
Bone Marrow / metabolism
Cohort Studies
DNA Primers / chemistry
Enzyme-Linked Immunosorbent Assay
Female
Gene Expression Regulation, Leukemic / physiology*
Humans
Immunoenzyme Techniques
Leukemia, Myeloid, Acute / classification
Leukemia, Myeloid, Acute / genetics*
Leukemia, Myeloid, Acute / metabolism
Male
Middle Aged
Prognosis
RNA, Messenger / genetics
RNA, Messenger / metabolism
Receptor, TIE-2 / genetics*
Receptor, TIE-2 / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Survival Rate
Vascular Endothelial Growth Factor A / genetics*
Vascular Endothelial Growth Factor A / metabolism
Vascular Endothelial Growth Factor C / genetics*
Vascular Endothelial Growth Factor C / metabolism

Substances

ANGPT1 protein, human
Angiopoietin-1
Angiopoietin-2
DNA Primers
RNA, Messenger
VEGFA protein, human
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factor C
Receptor, TIE-2