Abstract
Vascular smooth muscle cells (VSMCs) play a pivotal role in vascular injury through proliferation and migration. Pro-inflammatory cytokines and cyclooxygenase (COX)-2 and nitric oxide synthase (NOS) are highly associated with the pathogenesis of VSMCs. We investigated the effect of bioactive ceramics on the expression of inflammatory cytokines, COX-2, and inducible NOS (iNOS) induced by phorbol 12-myristate 13-acetate (PMA) in rat VSMCs. The ceramics inhibited mRNA expression of IL-1beta, TNF-alpha, IL-6, COX-2, and iNOS. Prostaglandin release was also diminished by the ceramics. The bioactive ceramics effect on cytokines, COX-2, and iNOS expression was achieved by inhibition of NF-kappaB activity. Interestingly, the ceramics-induced up-regulation of expression of endothelial NOS resulted in an increase of nitric oxide production. Thus, bioactive ceramics may have dual effects on the pathogenesis of VSMCs by regulation of NF-kappaB activity and NO production.
Publication types
-
Evaluation Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Anti-Inflammatory Agents / pharmacology
-
Cells, Cultured
-
Ceramics / pharmacology*
-
Coronary Artery Disease / genetics
-
Coronary Artery Disease / metabolism
-
Coronary Artery Disease / pathology*
-
Cyclooxygenase 2 / genetics
-
Cyclooxygenase 2 / metabolism
-
Gene Expression Regulation / drug effects
-
Inflammation Mediators / metabolism
-
Muscle, Smooth, Vascular / drug effects*
-
Muscle, Smooth, Vascular / metabolism
-
Muscle, Smooth, Vascular / pathology
-
Myocytes, Smooth Muscle / drug effects*
-
Myocytes, Smooth Muscle / metabolism
-
Myocytes, Smooth Muscle / pathology
-
NF-kappa B / metabolism
-
Nitric Oxide / metabolism
-
Nitric Oxide Synthase Type II / genetics
-
Nitric Oxide Synthase Type II / metabolism
-
Prostaglandins F / metabolism
-
Rats
-
Tetradecanoylphorbol Acetate / pharmacology*
Substances
-
Anti-Inflammatory Agents
-
Inflammation Mediators
-
NF-kappa B
-
Prostaglandins F
-
Nitric Oxide
-
Nitric Oxide Synthase Type II
-
Nos2 protein, rat
-
Cyclooxygenase 2
-
Ptgs2 protein, rat
-
Tetradecanoylphorbol Acetate
-
prostaglandin F1