Objective: To optimize the extraction process of total saopnins from Paris polyphylla Smith.
Methods: The single factor test and orthogonal experiment were used to determine the optimum extraction process.
Results: The optimum extraction process was obtained as follows: the plant materials were extracted with 70% ethanol twice, respectively with 10BV for 2 hours and then with 8BV the solvents for 1.5 hours. The yield of total saponins could be up to 4.24% and the total extraction rate of Paris polyphylla I and Paris polyphylla II was 93.28%.
Conclusion: The optimum process obtained is steady, reasonable and feasible.