Abstract
Members of the YidC/Oxa1/Alb3 protein family function in the biogenesis of membrane proteins in bacteria, mitochondria and chloroplasts. In Escherichia coli, YidC plays a key role in the integration and assembly of many inner membrane proteins. Interestingly, YidC functions both in concert with the Sec-translocon and as a separate insertase independent of the translocon. Mitochondria of higher eukaryotes contain two distant homologues of YidC: Oxa1 and Cox18/Oxa2. Oxa1 is required for the insertion of membrane proteins into the mitochondrial inner membrane. Cox18/Oxa2 plays a poorly defined role in the biogenesis of the cytochrome c oxidase complex. Employing a genetic complementation approach by expressing the conserved region of yeast Cox18 in E. coli, we show here that Cox18 is able to complement the essential Sec-independent function of YidC. This identifies Cox18 as a bona fide member of the YidC/Oxa1/Alb3 family.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphatases / metabolism
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Bacterial Proteins / metabolism
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Electron Transport Complex IV / metabolism
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Escherichia coli / genetics
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Escherichia coli / metabolism*
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism*
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Genetic Complementation Test
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Membrane Proteins / metabolism*
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Membrane Transport Proteins / metabolism*
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Mitochondrial Proteins / metabolism
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Models, Biological
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Nuclear Proteins / metabolism
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SEC Translocation Channels
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins / metabolism*
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SecA Proteins
Substances
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Bacterial Proteins
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COX18 protein, S cerevisiae
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Escherichia coli Proteins
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Membrane Proteins
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Membrane Transport Proteins
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Mitochondrial Proteins
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Nuclear Proteins
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OXA1 protein
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SEC Translocation Channels
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Saccharomyces cerevisiae Proteins
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YIDC protein, E coli
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Electron Transport Complex IV
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Adenosine Triphosphatases
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SecA Proteins